1 The Kidney Disease Improving Global Outcome (KDIGO) working group suggested a definition based on a plasma creatinine (Cr) level or a urinary volume, which is increase of Cr by no less than 1.5 times within 7 days or by at least 0.3mg per deciliter within 48 hours after exposure to CM, or decrease of urinary volume to less than 0.5 mL per kilogram of body weight per hour that persists for at least 6 hours after exposure. Keywords: icariin, contrast medium, acute kidney injury, competing endogenous RNA, non-coding RNAĬontrast induced acute kidney injury (CIAKI) is characterized by development of acute kidney injury (AKI) after intravascular administration of contrast medium (CM). The ceRNA network, involving Acot1, Cbwd1, Ly6i, Map3k14, Mettl2, Nyap1, Set, Tmem44 and Utp20, might partially contribute to the underlying mechanism of icariin protection by regulation of ERK1 and ERK2 cascade, MAPK signaling and NF-κB signaling. Among them, eight mRNAs (Acot1, Cbwd1, Ly6i, Map3k14, Mettl2b, Nyap1, Set and Utp20) were negatively correlated with renal function, while one mRNA (Tmem44) was positively correlated with renal function.Ĭonclusion: Icariin presented a protective effect against CIAKI. Further, two circRNAs, six lncRNAs, four miRNAs and nine mRNAs were selected as hub genes of the ceRNA network. Function annotation showed the ceRNA networks were enriched in ERK1 and ERK2 cascade, MAPK signaling and NF-κB signaling. Based on DEGs in icariin pretreated CIAKI rats, lncRNA- and circRNA-associated ceRNA networks were constructed, respectively. Results: Icariin protected against CIAKI in both in vivo and in vitro models. Association of hub genes in the ceRNA network and renal function was also examined. Expressions of hub genes were validated by PCR. By Cytoscape software, the competing endogenous RNA (ceRNA) network was constructed, and hub genes were selected. The function annotation of DEGs was analyzed by Metascape. The differentially expressed genes (DEGs) were screened and visualized by R software. Rat kidneys were collected for genome-wide sequencing. Methods: The effect of icariin was evaluated in both in vivo and in vitro CIAKI models. This study aimed to investigate the effect of icariin in CIAKI, as well as exploring the underlying mechanism from the aspect of interaction between protein-coding genes and non-coding RNAs. However, the role of icariin in contrast-induced acute kidney injury (CIAKI) is still unclear. *These authors contributed equally to this workĬorrespondence: Zena Huang, Yunkang School of Medicine and Health, Nanfang College, Guangzhou, People’s Republic of China, Tel +86-13570466614, Email Renyuan Li, Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of China, Tel +86-18926146852, Email īackground: Icariin presents protective effect in several kidney diseases. Yan Lin, 1, 2, * Gaofeng Zhu, 3, * Xiaoyong Li, 4, * Huaxiao Yu, 5 Yuhang Luo, 5 Jiaqiong Lin, 6 Renyuan Li, 7 Zena Huang 2ġDepartment of Nephrology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of China 2Yunkang School of Medicine and Health, Nanfang College, Guangzhou, People’s Republic of China 3Department of General Medicine, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, People’s Republic of China 4Department of General Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of China 5The Third Clinical School, Guangzhou Medical University, Guangzhou, People’s Republic of China 6Affiliated Dongguan Maternal and Child Healthcare Hospital, Southern Medical University, Guangzhou, People’s Republic of China 7Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of China

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